Influence of Non-enzymatic Antioxidants on Antioxidation Status in Acute Haemorrhagic Necrotizing Pancreatitis in Rat

The aim of the work was to assess the influence of some non-enzymatic antioxidants (uric acid, blood urea, bilirubin) on the antioxidant status of rat’s organism during the experimental acute haemorrhagic necrotising pancreatitis. The experiment was carried out on male Wistar rats, body weight 280-320 g, which were divided randomly into control and experimental groups. The control group (n=8) underwent laparotomy. The experimental group (n=24) was injected into pancreatic duct with natrium taurocholate to induce acute pancreatitis – according to the Heinkel and Aho method. Total antioxidation status in plasma was assessed according to the Benzie method (FRAP); total bilirubin, urea, and uric acid in plasma were assessed as described elsewhere. These parameters were examined in each group 3 h, 24 h, and 48 h after the experiment started. Simultaneously α-amylase activity (indicator of pancreatitis severity) was assessed in plasma. The obtained results were analysed statistically. Pancreatitis achieved its maximum severity 24 h after the beginning of the experiment and then decreased. After 48 h αamylase activity was only 2 times higher than that in the control group. FRAP maximum was noticed just after 3 h, then became lower and 48 h after the experiment onset its minimal value was 2 times lower than that of the control. Uric acid and bilirubin plasma concentration grew up 24 h after experiment beginning and next decreased. Only plasma urea concentration increased stably to the maximum value which was observed 24 h after the operation and after 48 h only slight decrease in its concentration was noticed. This phenomenon may be the result of parenchymatous organs damage occurring in acute haemorrhagic necrotising pancreatitis. It leads to interlobular and periadenous tissue necrosis as a consequence of lipid peroxidation. The obtained results showed the booster effect of non-enzymatic antioxidant agents on total antioxidant status during experimental pancreatitis. This effect correlated with pancreatitis severity which was evidenced by an increased αamylase activity in plasma.